Services and Products

Screening

Drug development into any disease area relies heavily upon the evaluation of molecular activity using in vitro screening assays prior to the progression of compounds to in vivo models. At this stage compound cellular responses can be determined for a wide range of properties.

GlycoMar offers a suite of cost effective primary screens, using cells from healthy human donor volunteers and cell lines.

Please click on the links for more details:

Assay	Description	Application	Cell type	Throughput
Cytokine profiling	Quantitative multiplex determination of cytokine release in response to pro-inflammatory stimulation.	Identification of both pro- and anti-inflammatory molecules	Human whole blood, PBMCs, or neutrophis, keratincytes, or endothelial cells	Low-Medium 96-well format
Elastase Release	Determination of enzyme release in response to pro-inflammatory stimulation.	Identification of anti-inflammatory molecules acting on neutrophils	Human neutrophils	Low-Medium 96-well format
Elastase Inhibition	Determination of enzyme activity in a cell-free assay.	Identification of elastase inhibitors	None	Low-Medium 96-well format
Leukocyte migration	Determination of adhesion & migration of leukocytes to endothelial cells.	Identification of inflammatory cell recruitment inhibitors	Human neutrophils and primary HUVECs	Low-Medium 24-well format
Leukocyte chemotaxis	Determination of leukocyte chemotaxis in response to well characterised chemotactic agents or test agents.	Identification of chemotaxis modulators	Human neutrophils	Low-Medium 24-well format
NF-kB Activation	Gene-reporter assay for the determination of transcription factor (NF-kB) activation.	Identification of both pro- and anti-inflammatory molecules	Lung carcinoma cell line (A549)	Low-Medium 96-well format
Oxidative burst	Determination of reactive oxygen & nitrogen species production in response to pro-inflammatory stimulus.	Identification of pro- and anti-inflammatory molecules	Human neutrophils and lymphocytes	Low-Medium 96-well format
Lipid peroxidation	Determination of lipid peroxidation products primarily found in plasma membranes but also detected in plasma	Identification of anti-oxidant molecules which prevent lipid peroxidation	None	Low-medium 96-well format
Activated Partial Thromboplastin Time (APTT)	Quantitative determination of anticoagulant activity.	Identification of anticoagulant molecules	Fresh human plasma	Medium – High Automated
Cytotoxicity	Determination of cytotoxic activity.	Identification of cytotoxic molecules	HeLa cells	Low-Medium 96-well format
Endotoxin	Quantitative determination of endotoxin using PyroGeneTM assay.	Confirmation that products are endotoxin-free, for QC purposes	None	Low-Medium 96-well format

Benefits and Problem solving

GlycoMar’s screening activity is supported by the progression of it’s own in house drug discovery molecules into in vivo inflammatory models for evaluation.
Technical interpretation from our drug development professionals is available.
Cost is negotiated with respect to sample numbers and turnaround time.
Flexibility is offered in all aspects of our screening packages on a client to client basis.
Other specialised contract glycobiology services are also available, such as purification and derivatization of molecules.
Quality is assured in all activities under the supervision of a designated Quality Manager.

Screening packages can be tailored to suit individual client requirements from small research groups to larger drug development organisations.

For more information contact us.