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Cytokine / Chemokine Screening – Release Assays
An early step in the inflammatory process is activation of inflammatory cells, which results in the release of inflammatory cytokines, such as tumour necrosis factor (TNF)-α and interleukin (IL)-1β. Inflammatory cytokines are central to many of the downstream events, such as leukocyte recruitment, which occur in an inflammatory response.
Assay Principle
GlycoMar’s cytokine/chemokine release assay measures the release of inflammatory cytokines and chemokines from freshly isolated human peripheral blood mononuclear cells (PBMCs) or neutrophils (alternative cell types available on request). The assay can be used to detect both pro-inflammatory and anti-inflammatory activity of test agents. In pro-inflammatory mode the assay looks for the up regulation of key cytokine markers released from cells treated with test agents only. In anti-inflammatory mode the assay uses well characterised inflammatory activators such as PHA, LPS, and IL-1β to stimulate cytokine and chemokine release and looks for down regulation of that release by test agents.
Cytokines and chemokines are determined by individual or multiplex (up to 9 analytes) ELISA; possible analytes include pIL-1β, IL-2, IL-6, IL-8, IL-10, IL-12, IL-13, IFNγ, GM-CSF, TNF-α (full list of analytes available on request).
Results
Results are reported as the amount of analyte released and calculated as percentage release relative to a standard control. Results are reported, summarised graphically, in a format agreed with the client.
Table 1. Cytokine release from human neutrophils stimulated with TNF-α in the presence and absence of test agents
Figure 1. Graphical presentation of cytokine release data from Table 1, relative to stimulated control cells
Figure 2. IL-6 release assay dose response of human PBMCs stimulated with IL-1β showing [Il-6] ± standard error (SEM)
GlycoMar is committed to ensuring quality in all of its activities through a GLP-based Quality Assurance system.
Sample Submission
Samples can be submitted to GlycoMar by prior arrangement, in a number of formats.
Pricing is dependant on sample numbers submitted and turnaround required.
Standard turnaround is 4 weeks from receipt, but faster turnaround is possible on request.
Please contact GlycoMar directly for a quotation, or to discuss your individual requirements:
Tel: +44 (0) 1631 559 368 E-mail: info@glycomar.com
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